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. Author manuscript; available in PMC: 2013 Oct 10.
Published in final edited form as: J Control Release. 2012 Jun 12;163(1):25–33. doi: 10.1016/j.jconrel.2012.06.007

Figure 3. Caco-2 monolayers as a model for transepithelial transport.

Figure 3

Caco-2 cells were grown on permeable membrane inserts (0.4-µm pore) at 1.5×105 cells/cm2. (A) Transepithelial electrical resistance (TEER) was measured to assess monolayer integrity. Cells were treated with TNF-α on Day 14. Data are means±S.E.M. (n=3). (B) Fluorescence microscopy of tight junctions immunolabeled with anti-occludin and TxR secondary antibody on Days 5 or 14 (low versus high TEER values, respectively). (C) Comparison of control versus TNF-α-activated cells stained for tight junctions as in (B). (D) Fluorescence microscopy comparing ICAM-1 immunostaining of TNF-α-activated, confluent Caco-2 cells either grown on coverslips or transwell inserts. The dashed lines mark the cell borders, observed by phase-contrast. Magnification bar=10 µm.