Table 3.
Transepithelial transport of α-Gal by anti-ICAM NCs
| Total NCs associated/cell |
Intracellular NCs/cell |
Transported NCs/cell |
% Transported |
Transported α-Gal molecules/cell × 105 |
pg transported/cell × 10−3 |
Papp (×10−8cm/s) |
|
|---|---|---|---|---|---|---|---|
| 3h | 663.4 ± 116.0 | 434.0 ± 76.8 | 243.6 ± 15.4 | 44.4 ± 6.7 | 1.8 ± 0.2 | 9.7 ± 1.2 | 8.1 ± 1.1 |
| 24 h | 2024.8 ± 409.3 | 1218.9 ± 255.6 | 806.9 ± 164.1 | 40.6 ± 2.0 | 3.9 ± 0.5 | 21.3 ± 2.5 | 1.9 ± 0.4 |
NCs = nanocarriers; α-Gal = α-galactosidase; Total NCs associated/cell = NCs in the cell fraction/number of cell + NCs transported/number of cell; % Transported = 100 × (NCs transported / Total NCs associated); Papp = apparent permeability coefficient (cm/s). See Methods for further description of these parameters. Data were obtained in quiescent Caco-2 cells, and represent means ± S.E.M. (n = 8 wells).