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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Fertil Steril. 2012 Jul 11;98(4):948–956.e1. doi: 10.1016/j.fertnstert.2012.06.009

Figure 3. In vitro TCDD exposure blocks progesterone induction of CB1-R mRNA expression in endometrial stromal cells obtained from control donors.

Figure 3

Stromal cells were isolated from control endometrial biopsies taken during the secretory phase (N=5) and cultured 4 days with estradiol + progesterone (E; 1nM; P; 500nM) with or without TCDD (10nM). CB1-R mRNA expression was robust in cells maintained in EP, while those cells treated with TCDD showed significantly reduced expression of this transcript (P< 0.001). qRT-PCR data, normalized to the housekeeping gene, was analyzed using the ΔΔCT [59] method and *** is indicative of P< 0.001.