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. Author manuscript; available in PMC: 2013 Sep 28.
Published in final edited form as: Mol Cell. 2012 Aug 9;47(6):839–850. doi: 10.1016/j.molcel.2012.07.002

Figure 4. The N-terminus of TIN2 mediates TIN2-TPP1 interaction.

Figure 4

A) Wildtype (Wild) and mutant TIN2-GFP proteins were transiently expressed with or without flag-TPP1 in HEK-293 cells, immunoprecipitated (IP) with anti-flag antibodies, and western blotted as indicated. Arrow indicates a non-specific band. B) Similar analysis of flag-tagged wildtype (Wild) and point mutants of TIN2 proteins in HEK-293 cells transiently co-expressing TPP1-V5. C) Analysis of cells co-expressing TRF1-V5 with flag-tagged wildtype (Wild) or mutant TIN2 proteins.