Fig. 4.

Selective inhibition of β1i, β5i or both using UK-101 or LKS01 does not block NF-κB DNA binding activity. (A) Immunoblotting results show that the stimulatory effect of TNFα (15 ng/ml, 90 min) on the nuclear translocation of NF-κB subunits p65 and p50 is not attenuated by pretreatment with UK-101 (1 μM) or LKS01 (0.2 μM), either individually or in combination, in H23 cells. (B) EMSA of nuclear extracts prepared from H23 cells showing that the TNFα-stimulated DNA binding activity of NF-κB is not diminished by pretreatment with UK-101 (1 μM) or LKS01 (0.2 μM), either individually or in combination, in H23 cells. The arrow marks the band that represents specific NF-κB binding.