Fig. 6.

Selected relaxation dispersion curves of Ca²⁺-bound S100B (± TRTK-12). Dispersion profiles of the backbone amides of Thr59 (a) and Thr82 (b) in the absence and presence of TRTK-12 are shown with data collected at 800 (red) and 600 (black) MHz. The relaxation dispersion curve of one of the terminal amide correlation of Ca²⁺-bound^D63NS100B (± TRTK-12) is shown in (c) with data collected at 800 (blue) and 600 (black) MHz. (d) Over lay of the canonical EF-hand of Ca²⁺-bound S100B (green) and Ca²⁺-bound^D63NS100B (purple). The RMSD of all atoms between the wild type and ^D63NS100B mutant canonical EF-hand is 0.283Ų.