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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Anal Biochem. 2012 Jul 31;430(1):4–15. doi: 10.1016/j.ab.2012.07.022

Fig. 7.

Fig. 7

A chromatogram from the GC-MS/MS analysis of AECK-DD in the brain of an untreated adult male Sprague-Dawley rat. SRM of m/z 328 to m/z 268 for AECK-DD (upper panel) and of m/z 330 to m/z 270 for 13C2-AECK-DD (lower panel) was performed. 13C2-AECK-DD was added at a final content of 50 pmol per 100 mg tissue. The smaller retention time of the PFB derivatives of AECK-DD and 13C2-AECK-DD (11.7 min versus 12.2 min) are due to the shortening of GC column compared to previous analyses (e.g., Fig. 6).