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. 2012 Sep 25;2012:745962. doi: 10.1155/2012/745962

Figure 2.

Figure 2

Internalization efficiency by DC2.4 cells is comparable between native and fixed OVA. (a) Labeling-efficiency with AF647 was equal between native and fixed OVA. Native and fixed-OVA were labeled with AF647 and graded doses were analyzed for their relative fluorescence incorporation. (b) DC2.4 cells were incubated with 1 mg/mL AF647-labeled native OVA or fixed OVA for the indicated times and internalization was analyzed by flow cytometer. (- - - -): native OVA, (—): fixed OVA. Gray line: unpulsed DC2.4 cells. Note: MFI = 114 (5 min), 159 (10 min), and 306 (15 min) with normal OVA and 77.3 (5 min), 160 (10 min), and 310 (15 min) with fixed OVA. The results were confirmed in two independent experiments.