92 |
AGTCGGCAGCACATACACAC |
vwf cloning, assembly of fragment 1 (EcoRI-BstBI) |
93 |
ATCCGGACAGGTCAGTTCAC |
vwf cloning, assembly of fragment 1 (EcoRI-BstBI) |
94 |
CCTGCAGCTTAAACCCAAAG |
vwf cloning, assembly of fragment 2 (BstBI-AvaI) |
95 |
AAAGCTTCATCGTCCAGCTC |
vwf cloning, assembly of fragment 2 (BstBI-AvaI) |
96 |
CTGTTGACGGCAAGTGCTAA |
vwf cloning, assembly of fragment 3 (AvaI-SbfI) |
97 |
TCTCCTGATGCTGGACACAC |
vwf cloning, assembly of fragment 3 (AvaI-SbfI) |
98 |
GACGGCAGTGTAACGACAGA |
vwf cloning, assembly of fragment 4 (SbfI-ApaLI) |
99 |
CCTGCAAGAGAGCCGATAAC |
vwf cloning, assembly of fragment 4 (SbfI-ApaLI) |
116 |
TGCGTGCTGAATCAAACTGT |
vwf cloning, 3′ RACE (ApaLI-SpeI), SpeI vector derived |
128 |
AGTCGCCAGGGAATTCATAA |
vwf cloning, 5′ RACE (NotI-EcoRI), NotI vector derived |
130 |
TTTGATTGACATTTTTATTTATTGTAGTTTA |
vwf cloning, amplification of 3′ UTR |
543 |
gatttaggtgacactatagCGACATGCAAGTGCAGAAGT |
424 bp vwf riboprobe (exon 28) with SP6 promoter overhang |
544 |
taatacgactcactatagggGCTGGGTTTTGCTGTAGGAG |
424 bp vwf riboprobe (exon 28) with T7 promoter overhang |
545 |
gatttaggtgacactatagGGAGTTATCGGCTCTCTTGC |
441 bp vwf riboprobe (exons 47–52) with SP6 promoter overhang |
546 |
taatacgactcactatagggACACAGACTTGCTGCCACAC |
441 bp vwf riboprobe (exons 47–52) with T7 promoter overhang |
|
|
CTCGAGAGAATTCCACCACACTGGACTAGTGGATC |
XhoI-PmeI human VWF linker sequence (includes Myc/HIS tag) |
|
CGAGCTCGGTACCAAGCTTGGGCCCGAACAAAAAC |
|
TCATCTCAGAAGAGGATCTGAATAGCGCCGTCGAC |
|
CATCATCATCATCATCATTGAGTTTAAAC |