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. 2012 Sep 24;2012:214209. doi: 10.1155/2012/214209

Table 1.

List of primers and sequences.

Reference number Sequence Description
92 AGTCGGCAGCACATACACAC vwf cloning, assembly of fragment 1 (EcoRI-BstBI)
93 ATCCGGACAGGTCAGTTCAC vwf cloning, assembly of fragment 1 (EcoRI-BstBI)
94 CCTGCAGCTTAAACCCAAAG vwf cloning, assembly of fragment 2 (BstBI-AvaI)
95 AAAGCTTCATCGTCCAGCTC vwf cloning, assembly of fragment 2 (BstBI-AvaI)
96 CTGTTGACGGCAAGTGCTAA vwf cloning, assembly of fragment 3 (AvaI-SbfI)
97 TCTCCTGATGCTGGACACAC vwf cloning, assembly of fragment 3 (AvaI-SbfI)
98 GACGGCAGTGTAACGACAGA vwf cloning, assembly of fragment 4 (SbfI-ApaLI)
99 CCTGCAAGAGAGCCGATAAC vwf cloning, assembly of fragment 4 (SbfI-ApaLI)
116 TGCGTGCTGAATCAAACTGT vwf cloning, 3′ RACE (ApaLI-SpeI), SpeI vector derived
128 AGTCGCCAGGGAATTCATAA vwf cloning, 5′ RACE (NotI-EcoRI), NotI vector derived
130 TTTGATTGACATTTTTATTTATTGTAGTTTA vwf cloning, amplification of 3′ UTR
543 gatttaggtgacactatagCGACATGCAAGTGCAGAAGT 424 bp vwf riboprobe (exon 28) with SP6 promoter overhang
544 taatacgactcactatagggGCTGGGTTTTGCTGTAGGAG 424 bp vwf riboprobe (exon 28) with T7 promoter overhang
545 gatttaggtgacactatagGGAGTTATCGGCTCTCTTGC 441 bp vwf riboprobe (exons 47–52) with SP6 promoter overhang
546 taatacgactcactatagggACACAGACTTGCTGCCACAC 441 bp vwf riboprobe (exons 47–52) with T7 promoter overhang
CTCGAGAGAATTCCACCACACTGGACTAGTGGATC XhoI-PmeI human VWF linker sequence (includes Myc/HIS tag)
CGAGCTCGGTACCAAGCTTGGGCCCGAACAAAAAC
TCATCTCAGAAGAGGATCTGAATAGCGCCGTCGAC
CATCATCATCATCATCATTGAGTTTAAAC