. Author manuscript; available in PMC: 2012 Oct 2.

Published in final edited form as: Mol Biosyst. 2010 Mar 29;6(9):1583–1591. doi: 10.1039/c002259d

Table 1.

Apparent K_d values for the BG-A monoclonal antibodies.

Ligand	Density ratio	81FR2.2	Apparent K_d values (nM)^a			B480
Ligand	Density ratio	81FR2.2	HE-193	HE-195	Z2A	B480
Adi-04	1:0	-	-	-	0.69±0.31	0.92±0.17
	1:1	-	-	-	2.0±1.0	ND^a
	1:3	-	-	-	ND^a	ND^a
	1:7	-	-	-	ND^a	ND^a
Adi-17	1:0	-	ND^a	0.56±0.24	0.20±0.02	0.18±0.03
	1:1	-	-	1.1±0.6	0.37±0.10	0.66±0.29
	1:3	-	-	ND^a	0.78±0.30	1.8±0.4
	1:7	-	-	ND^a	1.4±0.6	ND^a
BG-A	1:0	0.80±0.25	0.31±0.04	0.066±0.021	0.11±0.01	0.03±0.003
	1:1	4.7±1.0	0.80±0.09	0.15±0.03	0.15±0.01	0.06±0.02
	1:3	8.3±2.6	1.9±0.5	0.18±0.05	0.22±0.03	0.11±0.02
	1:7	18±11	2.6±3.3	0.21±0.10	0.28±0.05	0.17±0.03
BG-A1	1:0	0.6±0.16	0.32±0.03	0.061±0.019	1.2±0.2	0.04±0.01
	1:1	7.2±3.0	3.0±1.2	0.12±0.03	2.6±0.3	0.12±0.03
	1:3	3.1±2.7	1.1±0.4	0.12±0.05	4.6±1.0	0.39±0.11
	1:7	11±2	ND^a	0.22±0.06	5.5±1.6	0.34±0.11
Forssman Di-04	1:0	-	-	-	0.42±0.14	-
	1:1	-	-	-	0.95±0.44	-
	1:3	-	-	-	2.5±1.5	-
	1:7	-	-	-	ND^a	-
Forssman Di-21	1:0	-	-	-	0.15±0.01	-
	1:1	-	-	-	0.23±0.03	-
	1:3	-	-	-	0.35±0.06	-
	1:7	-	-	-	0.45±0.12	-

ND indicates that the antibody did bind but the affinity was too weak to determine the app K_d. “-” = no binding.