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Flat-mounted retinas labeled with anti-cleaved Caspase-3 antibody (green) in different RGC-depleted retinas at P15. The 95% Atoh7−/− (C) and 99% Atoh7−/−; Pou4F2−/− (D) RGC-depleted retinas have abundant Caspase3-positive cells in the retinal margin that are not observed in the control (A) or Pou4F2−/− (B) retinas. Scale bar, 200 μm.
