Figure 7.

In situ hybridization showing elevated expression of Shh, Patched1, and Patched2 in the retinal margin of RGC-depleted retinas at P15. Elevated expressions of these genes are obvious in ATOH7-deficient (C, G, K) and ATOH7/POU4F2-deficient (D, H, L) retinas, but less clear in the Pou4F2-deficient retinas (B, F, J). Transcripts of these genes are downregulated in wild type at this stage (A, E, I). Note that elevated Ptch1 does not continue into the planoretinal junction and the pars plana (arrow in G). Scale bar, 50 μm.