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Acacetin suppressed eosinophil adhesion to BEAS-2B cells. HL-60 cells were treated with calcein-AM and added to BEAS-2B cells, and the results were observed using fluorescence microscopy. Note the adherence of HL-60 cells to normal (a) and TNF-α-activated BEAS-2B cells (b). Pretreatment of HL-60 cells with 3 μM acacetin (c), 10 μM acacetin (d), 30 μM acacetin (e), and 100 μM acacetin (f) reduced the adherence of HL-60 cells to BEAS-2B cells in a dose-dependent manner.
