Fig 6. Disease in susceptible mice is associated with neutrophil accumulation; macrophages do not participate in disease development.

A) Kidneys were harvested from mice at day 21 after injection of SLE serum “A”. nd= not determined. Dotted line represents the mean value obtained in susceptible mice given normal human serum and harvested at day 10 (top panel) or day 14 (middle and bottom panels). Neutrophil, macrophage and T cell infiltration were quantified by immunohistochemistry. Mice treated with anti-GBM serum are included as a positive control. Mean ±SEM is shown. *p<0.05, **p<0.01 compared to IIA⁺IIIIB⁺γ^−/− injected with SLE sera. n=3–7 mice per group. nd= not determined. Animals were treated with liposomes that contained clodronate or PBS and proteinuria was evaluated at indicated times in IIA⁺γ^−/− Mac-1^−/− (B) and IIA⁺γ^−/− (C) mice. ns= not significant, *p<0.05.