Differential Immune Response Marker Gene Activation by the 12–Amino Acid CLV3p and the 13–Amino Acid CLV3p.
Immune response marker genes, FRK1 (At2g19190) (A) and WRKY30 (At5g24110) (B), were activated by the 12–amino acid (aa) CLV3p but not the 13–amino acid CLV3p. Seven-day-old seedlings of Col-0 and the fls2 mutant grown in liquid culture were analyzed by qRT-PCR after treatment with 20 μM peptides for 1 h. The expression of each gene was normalized by ACT2 (At3g18780). Error bars indicate sd (n = 3).