Differential CLV3p and flg22 Activities in Seedling Assays.
(A) Root growth inhibition by the 12–amino acid CLV3p and the 13–amino acid CVL3p. Seedlings were grown in a liquid medium without (Control) or with various concentrations of the 12–amino acid CLV3p (square) or the 13–amino acid CLV3p (triangle). Primary root length was measured after 14 d. Error bars indicate sd (n = 10).
(B) Flg22 but not CLV3p triggers whole-seedling growth suppression. Ler (top panel) or clv2-1 (bottom panel) seedlings were grown in a liquid medium with 1 μM 12–amino acid (aa) CLV3p, 1 μM 13–amino acid CLV3p, or 10 nM flg22 for 9 d. The differential flg22 and CLV3p activities in whole-seedling suppression are best illustrated in the clv2 mutant. Bars = 1 cm.