Figure 3.

Sensitive MCLV3p Activation of Immune Response Marker Genes after Repurification.

(A) Repurified MCLV3p from two independent batches activated immune marker genes, FRK1, WRKY30, and PEP3 (At5g64905), in Col-0 seedlings grown in liquid culture. The treatment was without (Control) or with 1 μM MCLV3p for 1 h.

(B) Immune response marker gene activation by nanomolar range of MCLV3p in the mesophyll protoplast assay. Protoplasts were collected after treatment without (Control) or with 20 or 100 nM of MCLV3p for 1 h.

Expression of each gene was analyzed by qRT-PCR and normalized by ACT2. Error bars indicate sd (n = 3).