Figure 5.

Flg22 and MCLV3p Exhibit Distinct pH Optima in Immune Response Marker Gene Activation.

Immune response marker gene activation by 1 nM flg22 (A) or 1 μM MCLV3p (B) in Col-0 and fls2 seedlings grown in liquid culture. Seven-day-old seedlings grown in liquid medium were treated with 1 nM flg22 (A) or 1 μM MCLV3p (B) for 1 h in various pH ranges. The expression of each gene was normalized by ACT2. Relative fold activation was normalized by the control level without peptide treatment in each pH. Error bars indicate sd (n = 3).