Analysis of Pst DC3000 Infection in the SAM Regions of Seedlings Grown in Liquid Culture by Scanning Electron Microscopy.
Scanning electron microscopy analyses were performed with tissue cross sections.
(A) Immune protection of the Ler SAM area. A cross section through the SAM area is shown. Multiple cell layers below the SAM did not reveal any Pst DC3000 bacteria at 4 DAC. Bacteria were observed outside the dome.
(B) Compromised immune protection in the clv3 SAM area. Pst DC3000 colonized in the SAM and the cells below the SAM (4 DAC). Bars in (A) and (B) = 10 μm.
(C) Magnified image of the selected regions (white dot box) in (B) showing bacterial colonization. Bar = 5 μm.