Figure 3.

SDG724 Acts as a SET Domain–Containing Histone Methyltransferase.

(A) and (B) In vitro HMTase assay analyses. Calf thymus core histones (A) and oligonucleosomes (B) were used as substrates, and tritium-labeled SAM was used as methyl donor. Proteins analyzed for enzyme activity are pointed out with arrows. Top panel: Coomassie blue–stained protein gel. Bottom panel: Corresponding autoradiograph.

(C) Analysis of the global levels of H3K36me1/2/3, H3K4me1/2/3, and H3K9me2 markers in wild-type (WT), lvp1-1, and lvp1-2 plants using H3K methylation tag-specific antibodies. Histone-enriched proteins were extracted from leaves of 50-d-old plants grown in Beijing under natural LD conditions. For each immunoblot, the same membrane was stripped and reblotted with an antibody against H3 as a loading control.