Table 3. Complementation of glc Semisterility Phenotype by Genomic DNA of 450.2 and 450.3.
| Genotype | Wild-Type–Looking Seeds | Aborted Ovules | Total | Seed Set (%) | P Value (Fisher’s Exact Test) |
|---|---|---|---|---|---|
| glc; 450.2 (HYGR+) | 374 | 131 | 505 | 74.1 | 1.26e−16 |
| glc; 450.3 (HYGR+) | 395 | 149 | 544 | 72.6 | 2.49e−15 |
| glc (self) | 288 | 293 | 580 | 49.7 | N.A. |
| glc;450.2 (HYGR-) | 233 | 284 | 517 | 45.1 | 0.13 (N.S.) |
| glc;450.3 (HYGR-) | 290 | 279 | 569 | 51 | 0.68 (N.S.) |
glc plants were transformed with pCAMBIA1300 binary vector cloned with genomic DNA of 450.2 or 450.3. Seed set was scored in at least 10 siliques of T2 plants, which were genotyped for the presence/absence of the HYG resistance gene, which is linked to the genomic cloned insert. N.A., not applicable; N.S., not significant.