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. 2012 Aug 7;24(8):3264–3277. doi: 10.1105/tpc.112.096420

Table 4. Complementation of glc kanr/kans Distorted Ratio by cDNAs of 450.1, 450.2, and 450.3 Operating under the DD65 CC-Specific Promoter.

Genotype kanr kans Ratio kanr/kans P Value (Fisher’s Exact Test)
glc; 450.1 159 (73.9%) 56 (26.1%) 2.8:1 4.88e−15
glc; 450.2 140 (72.9%) 52 (27.1%) 2.7:1 1.4oe−13
glc; 450.3 141 (73.8%) 50 (26.2%) 2.8:1 4.61e−14
glc (self) 75 (36.2%) 132 (63.8%) 0.57:1 N.A.

The pB7WG2 binary vector was modified and its original 35S promoter was swapped with the DD65 CC-specific promoter, then cloned with cDNAs of 450.1, 450.2, and 450.3 and transformed to glc plants. The segregation of the linked nptll gene conferring kanr was scored in T2 seedlings grown on kan-MS medium. N.A., not applicable.