RGA Impairs the Activation of miR172 and SOC1 through SPL9.
(A) RGA interferes with the activation of SOC1 and MIR172b. We analyzed 30-d-old short-day–grown wild-type (WT), ProSPL9:rSPL9, and ProRGA:RGAd17 ProSPL9:rSPL9 plants. Error bars represent ±se (n = 3).
(B) and (C) Expression of SOC1
(B) and MIR172b
(C) in the PAC-treated wild-type, ProSPL9:rSPL9, and Pro35S:MIR156 plants. We collected 20-d-old short-day–grown seedlings 2 d after treatment. Error bars represent ±se (n = 3).
(D) Inducible expression of MIR172b. We sprayed 10-d-old long-day–grown ProSPL9:rSPL9-GR seedlings with DEX, DEX + PAC, or ethanol (mock) for 12 h. Error bars represent ±se (n = 3).