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Expression Analysis of Monolignol Biosynthesis Genes.
Total RNAs were prepared from wild-type (Ler) and lig mutant seedlings grown at 18 or 28°C for 5 d, and the expression of PAL1, CCR1, CCR2, CAD-C, and CAD-D was analyzed by RT-PCR. Amplification of UBQ is shown as a loading control. Similar experiments were performed four times with similar results.
