Table 1. Effect of the lig Mutation and the Addition of GlcNAc and GalNAc on the Contents of UDP-GlcNAc and UDP-GalNAc.
| Temperature | Addition |
UDP-GlcNAc (nmol/g) |
UDP-GalNAc (nmol/g) |
||
|---|---|---|---|---|---|
| Ler | lig | Ler | lig | ||
| 18°C, 8 d | None | 10.2 ± 0.5 | 14.5 ± 2.0 | 4.3 ± 0.4 | 5.7 ± 2.6 |
| 18°C, 5 d/28°C, 3 d | None | 9.4 ± 0.5 | 3.1 ± 0.6* | 3.5 ± 0.4 | 1.9 ± 0.5 |
| 18°C, 5 d/28°C, 4 d | GlcNAc | 25.0 ± 3.5 | 29.9 ± 8.5 | 7.8 ± 1.4 | 9.7 ± 3.7 |
| 18°C, 5 d/28°C, 4 d | GalNAc | 51.1 ± 13.3 | 55.8 ± 15.5 | 17.3 ± 8.1 | 19.9 ± 4.5 |
Seedlings of the wild type (Ler) and lig mutant that had been grown at 18°C for 8 d, or at 18°C for 5 d and then at 28°C for 3 d or 4 d. GlcNAc and GalNAc were administered at the final concentration of 10 µM. Nucleotide sugars were extracted from the root tissues and analyzed by HPLC for measurement of UDP-GlcNAc and UDP-GalNAc. The data represent average values per gram fresh weight obtained from three (top two rows) or two (bottom two rows) biological repeats with the mean se. An asterisk indicates a significant difference from the wild-type value under the same condition (Student’s t test, P < 0.01).