Figure 2.

G6PD Activity and H₂O₂ Levels Are Altered in ASKα Activity Mutants upon Salt Stress.

Four-week-old soil-grown Arabidopsis plants were watered with 150 mM NaCl for 3 weeks. The same plant material was used for (A) to (C). Analyses were performed with pools of 20 plants on three independent salt watering experiments. Data are means ± relative sd. Controls for each genotype were normalized to one ([A] and [B]).

(A) Total G6PD activity in leaf extracts of wild-type Col-0, askα, and ASKα overexpressor lines OE#4 and OE#5 grown under normal and high salinity conditions.

(B) H₂O₂ levels. H₂O₂ content was assessed spectrophotometrically in Col-0 and ASKα activity mutants.

(C) Total G6PD levels. Top lane: Total G6PD was immunoprecipitated from 50 µg protein extract and subsequently detected by immunoblot analysis using a polyclonal G6PD antibody. Bottom lane: CBB-stained gel.