ASKα Regulates Salt Stress Tolerance by Phosphorylating G6PD6.
(A) Phosphorylation of G6PD6 is important for salt stress tolerance. Germination efficiency of Col-0, askα, and askα mutants transformed with either G6PD6 or G6PD6 T467E on half-strength MS plates supplemented with 100 mM NaCl. Data are means ± relative sd of three independent biological replicates with at least 75 plants each. Asterisks indicate a significant difference compared with askα under stress conditions (*P < 0.05, **P < 0.01, and ***P < 0.005) using Student’s t test for pairwise comparison. WT, wild type.
(B) ASKα and G6PD6 function in the same signaling pathway. Survival rate of Col-0, askα, g6pd6, and askα g6pd6 double knockout plants on medium supplemented with 200 mM NaCl. Data are means ± relative sd of three independent biological experiments with at least 75 plants each.
(C) Model: ASKα-mediated phosphorylation of G6PD6 contributes to maintaining cellular redox balance under high salinity conditions.