Figure 4.

Sl-BSL1 and P. infestans AVR2 Colocalize and Accumulate around Haustoria in Planta.

(A) Transient expression of SlBSL1:RFP in N. benthamiana reveals that Sl-BSL1 is excluded from the nucleus and localizes at the cell periphery and within the cytoplasm.

(B) Transient expression of GFP:AVR2 and SlBSL1:RFP fusion proteins revealed similar patterns of localization for the two markers, accumulating at the cell periphery, as shown by overlapping peaks in fluorescence intensity. GFP:AVR2 was also detected in the nucleus.

(C) GFP:AVR2 and SlBSL1:RFP localize strongly at sites of P. infestans haustorial penetration in N. benthamiana.

Pictures were taken at 3 DAI. Bars + 10 µm.