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Immunoblots Showing That Five of the 13 AVR2 Family Members Associate with Sl-BSL1 and Activate R2-Mediated Recognition.
FLAG-tagged protein fusions of AVR2 family members were transiently expressed with pK7WGF2 or SlBSL1:GFP in N. benthamiana. Immunoprecipitates obtained with anti-FLAG or anti-GFP antiserum and total protein extracts were immunoblotted with appropriate antisera. IP, immunoprecipitate; PS, Ponceau stain.
[See online article for color version of this figure.]
