Immunoblots Showing That AVR2 Expression Is Required for Interaction of Sl-BSL1 with R2.
FLAG:AVR2, SlBSL1:myc, and GFP:SdR2 were transiently expressed in combination in N. benthamiana. Immunoprecipitates obtained with anti-FLAG or anti-GFP antiserum, and total protein extracts were immunoblotted with appropriate antisera. IP, immunoprecipitate; PS, Ponceau stain; Rubisco, ribulose-1,5-bisphosphate carboxylase/oxygenase.
[See online article for color version of this figure.]