Figure 4.

The rbcL Transcript Is Translated in raf1-1.

(A) Total polysomal extracts from the apical half of the second and third leaves of raf1-1 and wild-type (WT) 12-d-old seedlings were fractionated in 15 to 55% Suc gradients. RNA was extracted from 12 fractions of equal volume and analyzed by gel blot. EtBr, ethidium bromide.

(B) In vivo protein synthesis in 10-d-old leaves of wild-type, raf1-1, and bsd2 seedlings. [³⁵S]Met was incorporated in the presence of cycloheximide, as described in Methods. Total proteins from equal surface areas surrounding the perforations used to introduce the radiolabel were analyzed by SDS-PAGE. The left panel shows autoradiography; the right panel shows Coomassie blue staining of the same gel.

[See online article for color version of this figure.]