Figure 5.

Expression Pattern of O1.

(A) RNA expression level of O1 in various tissues. Ubiquitin was used as an internal control. For each RNA sample, three technical replicates were performed. Representative results from two biological replicates are shown. Error bars represent sd. Transcript abundance is indicated relative to tassel.

(B) Expression profiles of O1 during maize kernel development. Ubiquitin was used an internal control. For each RNA sample, three technical replicates were performed. Representative results from two biological replicates are shown. Error bars represent sd. Transcript abundance is indicated relative to 24-DAP kernels.

(C) Immunoblot analysis of O1 protein accumulation during kernel development.