Figure 8.

ER Streaming Is Suppressed in the Presence of a Dominant-Negative O1 Tail Construct Fused to eYFP.

(A) to (D) N. benthamiana leaves were infiltrated with the ER marker, mCherry-HDEL, alone.

(A) and (B) The first (A) and last frame (B) of a 50-frame 80-s movie (see Supplemental Movie 2 online).

(C) Merger of the first (colored red) and last frame (colored green) showing the total motility pattern.

(D) Three random, successive frames are colored green, red, and blue, respectively, and then overlapped together to generate an integrity image.

(A’) to (D’) N. benthamiana leaves were infiltrated with the ER marker, mCherry-HDEL, with eYFP-O1t. One image was acquired including the two channels to ensure that both reporters were present in the same cell, and a time-lapse movie was acquired using only the mCherry channel.

(A’) and (B’) The first (A’) and last frame (B’) of a 50-frame 80-s movie (see Supplemental Movie 4 online).

(C’) Merger of the first (colored red) and last frame (colored green) showing total motility pattern.

(D’) Three random, successive frames are colored green, red, and blue, respectively, and then overlapped together to generate an integrity image.

The brighter color indicates the slower movement. Bars = 10 μm.