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. 2012 Aug 31;24(8):3463–3481. doi: 10.1105/tpc.112.101758

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© 2012 American Society of Plant Biologists. All rights reserved.

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Figure 3. — Maize Mesophyll Protoplasts Coexpressing mCFP:ZmSYP121-Sp2 and mYFP:ZmPIP2;5 Phenocopies the Lower Plasma Membrane Water Permeability (P_f) of Arabidopsis syp121-1 Mesophyll Protoplasts.

(A) Relative distribution of P_f values of maize mesophyll protoplasts untransfected or (co)expressing mYFP:ZmPIP2;5, mYFP:ZmPIP2;5 and mCFP:ZmSYP121, mYFP:ZmPIP2;5 and mCFP:SYP121-Sp2, or mCFP:ZmSYP121.

(B) Median P_f values for each protoplast subpopulation shown in (A).

(C) Relative distribution of P_f values of mesophyll protoplasts from wild-type (WT) and syp121 Arabidopsis plants.

(D) Median P_f values for both Arabidopsis protoplast subpopulations.

Each bar represents the mean of 20 measurements. Error bars show 95% confidence interval. Statistical differences were inferred by the Kruskall-Wallis test followed by a Dunns test or Mann-Whitney U test. Medians that are significantly different (P < 0.05) are shown with different letters.