Table 1. FRET Efficiencies for the PIP/SYP121 Interaction Studies.

Protein	FRET Efficiency ± sd (%)	No. of Measurements	Cell Type
mCFP:ZmPIP1;2 + mYFP:ZmPIP2;5	25.7 ± 9.56	69	Maize protoplasts
mCFP:ZmSYP121 + mYFP:ZmPIP2;5	14.8 ± 3.12	35	Maize protoplasts
mCFP:ZmPIP2;5 + mYFP:ROP6	6.86 ± 9.26	20	Maize protoplasts
mCFP:ZmPIP1;2 + mYFP:ZmPIP2;5	23.9 ± 5.42	35	Tobacco epidermal cells
mCFP:ZmSYP121 + mYFP:ZmPIP2;5	14.1 ± 7.96	50	Tobacco epidermal cells

For each analysis, the average value of the FRET efficiency was determined as described in Methods.