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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Free Radic Biol Med. 2012 Jun 15;53(4):951–961. doi: 10.1016/j.freeradbiomed.2012.06.003

TABLE 1.

Influence of Initial Ascorbate Concentrations on •NO2-Induced Membrane Tyrosine Nitration

Aqueous Phase 3-Nitrotyrosine
100 μM AH2 —
50 μM AH2 + + +
25 μM AH2 + + +
10 μM AH2 + + + +

Red cell membranes (RCM) were covered with differing initial reduced ascorbate (AH2) concentrations and exposed to 4.5 ppm •NO2 in air for 30 min with cyclic tilting (2 min/side). Following exposure, RCM were washed, membrane proteins isolated, and the presence of 3-nitrotyrosine residues determined by Western analysis. As described in Methods, the extent of 3-NT formation is denoted as extensive (++++), significant (+++), modest (++), little (+), or none (-).