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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Free Radic Biol Med. 2012 Jun 15;53(4):951–961. doi: 10.1016/j.freeradbiomed.2012.06.003

TABLE 2.

Influence of Hydroxamic Acids and Related Compounds on •NO2-Induced Membrane Tyrosine Nitration

Aqueous Phase 3-Nitrotyrosine
50 μM DFO —
200 μM SHA —
200 μM BHA +
25 μM Hydroxyurea + +
200 μM HBED + + +
400 μM DTPA + + + +

Red cell membranes (RCM), adhered in petri dishes, were covered with various aqueous phase constituents and exposed to 4.5 ppm •NO2 in air for 30 min with cyclic tilting. Following exposure, RCM were washed, membrane proteins isolated, and the presence of 3-nitrotyrosine residues determined by Western analysis. As described in Methods, the extent of 3-NT formation is denoted as extensive (++++), significant (+++), modest (++), little (+), or none (-). 3-NT was undetectable during air-only exposures.