Skip to main content
. 2001 Jun 19;98(13):7086–7092. doi: 10.1073/pnas.111146798

Figure 2.

Figure 2

MAP2 is absent from dendritic spines. (A and B) Like the embryonic low-molecular weight variant MAP2c, high-molecular weight MAP2b is confined to the somatodendritic domain of hippocampal neurons and is absent from the axon (arrow). Shown are both a phase (A) and a fluorescence (B) image of a live neuron transfected with GFP-tagged MAP2b and kept in culture for 14 days. (Bar = 25 μm.) (C and D) This neuron transfected with MAP2b-GFP was maintained in culture for 4 weeks, by which time cells carry many dendritic spines. In the enlarged image (D) of the area outlined in C, the restriction of MAP2b-GFP fluorescence to microtubule bundles in the dendritic shaft is obvious. No fluorescence is detected in spine protuberances from the dendrite. (Bars: C = 15 μm; D = 2 μm.)