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Sensing the assembly of a 4-hairpin HCR using a CHA circuit. (a) Overall reaction of the 4-hairpin HCR reaction, where unlike H3 (see Figure 1a), the toehold (red) and branch-migration domain (green) locate on two separate molecules (H7 and H9, respectively), (b) Detailed reaction mechanism of the 4-hairpin HCR.
