Figure 3. Flt3L combined with Rapa promotes the production of CD4⁺CD25⁺Foxp3⁺ and CD8⁺CD25⁺Foxp3⁺ T cells.

Spleen cells isolated from mice treated with Flt3L/Rapa and other control mice at the time of rejection or at study endpoint (POD 100) were analyzed to determine the proportion of CD4⁺ CD25⁺ Foxp3⁺ and CD8⁺ CD25⁺ Foxp3⁺ T cells. (A, B) The expression of Foxp3 by CD4⁺ CD25⁺ and CD8⁺ CD25⁺ T cells was analyzed by flow cytometry after intracellular staining. The bar graph was a summary of percentages of CD4⁺ CD25⁺ Foxp3⁺ T cells and CD8⁺ CD25⁺ Foxp3⁺ T cells in the recipients. The data shown are representative of three independent experiments that yielded comparable results (* P<0.05).