(A) Sequence alignment of barnacle calcitonin isoform A and B. Sequences are from: decapods Homarus americanus ACX46386.1; copepods Caligus clemensi
[22], Lepeophtheirus salmonis ADD38663; branchiopod Daphnia pulex EFX90445; insects Tribolium castaneum EEZ99367, Drosophila melanogaster NP_523514, Anopheles gambiae XP_321755, Bombyx mori NP_001124379, Camponotus floridanus EFN61187 and Nasonia vitripennis XP_001599948. (B) Alignment of barnacle eclosion hormone. Sequences are from: decapods Callinectes sapidus CV224237, Penaeus monodon
[22], Marsupenaeus japonicus
[22]; branchiopods Daphnia pulex
[37], Triops cancriformis
[22]; insects Acromyrmex echinatior EGI68318, Anopheles gambiae XP_001230805, Drosophila melanogaster NP_524386, Bombyx mori NM_001043842 and Tribolium castaneum XP_969164. Asterisks “*” mark the six conserved Cysteine residues in EH.