Table 2. Stable carbon isotope analysis of the incorporation of 13C-labeled bicarbonate by the epibiotic microbial community on S. crosnieri setae.
Incubation Time (hours) | Tracer and Additional Energy Source | δ13C (‰)* | Average 13C/12C | % Enrichment |
0 | – | −41.3±0.2 | 0.01077±0.00002 | – |
48 | 13CO2 | 778±34 | 0.0200±0.0004 | 74±3 |
13CO2 + thiosulfate | 1851±68 | 0.0320±0.0008 | 176±7 |
δ 13C values were measured in triplicate and were expressed as the mean ± SD.
The 13C enrichment E (%) was calculated using the following equation: E = (Fsample/Fcontrol − 1) ×100 (%). Fsample and Fcontrol represent the isotopic abundance of 13C atoms of the epibionts before and after 13C labeling [F = (13C/12C)/(1+13C/12C)].