Table 3. Cell-specific incorporation of 13C-labeled bicarbonate by the Sulfurovum epibionts on S. crosnieri seta in the presence and the absence of thiosulfate according to FISH–Nano-SIMS.
Tracer | Location | Average 13C/12C | Enrichment (%) |
1* | 0.428±0.086 | 2713±588 | |
2* | 0.292±0.058 | 2019±435 | |
NaH13CO3 | 3* | 0.394±0.079 | 2551±552 |
+ | 4† | 0.135±0.027 | 1012±225 |
thiosulfate | 5† | 0.082±0.016 | 608±143 |
6† | 0.010±0.002 | −10±20 | |
7† | 0.011±0.002 | −5±20 | |
8† | 0.010±0.002 | −10±20 | |
1* | 0.009±0.002 | −15±20 | |
2* | 0.010±0.002 | −10±20 | |
NaH13CO3 | 3* | 0.011±0.002 | −5±20 |
4* | 0.009±0.002 | −15±20 | |
5* | 0.009±0.002 | −15±20 | |
6* | 0.010±0.002 | −10±20 | |
– | Control epibiont cells | 0.01077 | – |
The percent enrichment was estimated using the average 13C/12C ratio of bulk organic carbon of the epibiotic microbial community before the experiment shown in Table 2.
The filamentous cells were identified as Sulfurovum members by FISH.
The filamentous cells were not identified as the Sulfurovum members by FISH.