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. 2012 Oct 2;7(10):e45306. doi: 10.1371/journal.pone.0045306

Figure 2. Heating decreased protozoan numbers.

Figure 2

Soil microcosms were exposed to four different heating intensities (15°C, 60°C, 75°C or 90°C) for 24 h and subsequently amended with either P. fluorescens DSM 50090 or just with phosphate buffer. Culturable protozoa (heterotrophic flagellates: upper panel, naked amoebae: lower panel) were measured after 3 and 42 days. Heating above 60°C completely eliminated the protozoa, whereas amendment with P. fluorescens had no effect. We used a three-way ANOVA with sampling time and heating intensity as quantitative variables and Pseudomonas fluorescens DSM 50090 as qualitative variable to test the results. Data were log transformed prior to analysis to equalize variances. P-values <0.05 are shown.