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. 2012 Oct 2;7(10):e46866. doi: 10.1371/journal.pone.0046866

Figure 2. Caveolin 2 expression knockdown by shRNA.

Figure 2

A) ACHN cells were stably transfected with gene-specific shRNA to GAPDH, or CAV2 (CAV2–53 and CAV2–56). Quantitative real-time PCR was used to determine the relative amount of CAV2 mRNA in transfected and non-transfected ACHN cells. Results of triplicate samples are shown and are expressed relative to non-transfected ACHN cells. The asterisk denotes results that are significantly different from non-transfected ACHN cells (p<0.05, ANOVA followed by Dunnett's post hoc test). B) Whole-cell lysates from non-transfected and stably transfected ACHN cells were immunoblotted with anti-CAV2 antibody; anti-β-actin antibodies were used as a loading control. A representative immunoblot is shown.