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. 2012 Oct 2;7(10):e46866. doi: 10.1371/journal.pone.0046866

Figure 3. ε-toxin-induced cytotoxicity in caveolin-2-deficient cells.

Figure 3

ACHN cells (⧫), or ACHN cells stably transfected with gene-specific shRNA [GAPDH (▪), CAV2–53 (•) and CAV2–56 (▴)] were incubated with serial dilutions (15.6 to 1000 nM toxin) of purified ε-toxin at 37°C. Cell viability was assessed as described in the Materials and Methods. Results represent the mean and standard deviation of quadruplicate samples. The mean toxin dose needed to kill 50% of cells was calculated by non-linear regression analysis of results from at least three different experiments (60 nM, 55 nM, 158 nM, and 212 nM for wild-type, GAPDH-shRNA, CAV2–53, and CAV2–56, respectively). Values were compared by ANOVA followed by Dunnett's post hoc test. The ACHN cells transfected with CAV2-shRNA required a greater amount of toxin to kill 50% of the cells than was required to kill 50% of the parental ACHN cells or cells transfected with GAPDH-shRNA (P<0.05). The toxin dose required to kill 50% of cells transfected with GAPDH-shRNA was not significantly different than the dose required to kill 50% of wild-type ACHN cells.