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. 2012 Oct 2;7(10):e46866. doi: 10.1371/journal.pone.0046866

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A) Non-transfected ACHN cells, or cells transfected with shRNA specific for GAPDH or CAV2 were treated with GFP-labeled toxin. Whole cell lysates were analyzed by immunoblotting with GFP antibody. Non-transfected ACHN cells in the absence of toxin served as a control. A representative immunoblot is shown. B) Non-transfected ACHN cells, or cells transfected with a non-targeting shRNA or with CAV1-specific shRNA were treated with GFP-labeled toxin. Whole cell lysates were analyzed by immunoblotting with GFP antibody. Non-transfected ACHN cells in the absence of toxin served as a control. A representative immunoblot is shown.