Skip to main content
. 2012 Oct 2;7(10):e46773. doi: 10.1371/journal.pone.0046773

Figure 6. MiR-128 alone or together with miR-103 or miR-137 reduces endogenous NF1 protein levels in neurons.

Figure 6

(A) Representative images of transfected hippocampal neurons (green) stained with NF1 antibody (red). Merged images are shown in the third column of the panel. E16 murine hippocampal neurons were transfected with scramble or pri-mir-128 plasmids immediately after plating by using Lipofectamine 2000. Immunocytochemistry was carried out 40 hours after transfection. (B) Average decrease in NF1 protein levels. 80 neurons were analyzed per experiment. Data show the mean ± s.e.m from 6 independent experiments (*, P<0.05; **, P<0.01). (C) Freshly dissociated hippocampal neurons were transfected with Nf1 reporter vector plus antisense 2′-O-methyl inhibitors (miR-128 alone or together with miR-103 or miR-137) and assayed 48 hours later. Data show the mean ± s.e.m from 4 independent experiments (*, P<0.05).