Table 2. Primers used in this study.
| Primer Name | Sequence (5′ → 3′) |
| fabA upstream primers | |
| pfabA0 | cgggaatgaacgattacctg |
| pfabA-attB1a | GGGGACAAGTTTGTACAAAAAAGCAGGCTcatgaccggatcgccttcgaa |
| pfabA1-attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGTtctacgacaagggcggcaagc |
| pfabA2-attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGTggacgcgggaataaagtgaac |
| pfabA3-attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGTatctgttcgccggacactgtg |
| pfabA4-attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGTactttcaccgcaacgcaacag |
| pfabA4a-attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGTctgtgactttcaccgcaacg |
| pfabA5-attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGTtctatgactaggctgccgctg |
| pfabA6-attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGTcgacgccgatacaataacccg |
| pfabA7-attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGTgcgcgacggccgctggacgaa |
| pfabA8-attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGTccgccacaaccctgcagttca |
| pfabA9-attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGTgggatttttgaggagctcgc |
| pfabA10-attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGTatgaccaaacaacacgccttc |
| pfabA11-attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGTatcagcgatgtcggcggcaag |
| pPA1612-UP-attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGTcgccacctgctctacttca |
| fabA Δ30 | |
| PCRSOE-A | AGGTATCCGGTAAGCGGCAG |
| IR187SOE-B | TGTCCGGCGAACAGATGTTC |
| IR187SOE-C | GAACATCTGTTCGCCGGACATGACTAGGCTGCCGCTGCGA |
| PCRSOE-D | CGGTTCCTTTAGCAGCCCTT |
| PA1627 deletion | |
| 1627SOE-A | gtgatcagttgcagcatcaccg |
| 1627SOE-B | TCAGAGCGCTTTTGAAGCTAATTCGacccgggacagacccatgact |
| 1627SOE-C | AGGAACTTCAAGATCCCCAATTCGggaagaaacatccaatcatcggat |
| 1627SOE-D | accagcagtactaccaggaacc |
| PA4890 deletion | |
| 4890SOE-A | tgaaggattccgtctgcaagcc |
| 4890SOE-B | TCAGAGCGCTTTTGAAGCTAATTCGgaggacatacggcttcctttgg |
| 4890SOE-C | AGGAACTTCAAGATCCCCAATTCGgctgcgtttcatcatgatcggc |
| 4890SOE-D | tagttgaactccgcctcgccat |
| PA1539 deletion | |
| 1539SOE-A | gcagggtagtagttgtgcgaca |
| 1539SOE-B | TCAGAGCGCTTTTGAAGCTAATTCGtcgctcatgttccacctggttg |
| 1539SOE-C | AGGAACTTCAAGATCCCCAATTCGcgagtgacagctcgatgtcctt |
| 1539SOE-D | cgactaccgtttctgaatccgc |
| anr deletion | |
| anr-UpF-GWL | TACAAAAAAGCAGGCTttgacagggtgcgacaggta |
| anr-UpR-Gm | TCAGAGCGCTTTTGAAGCTAATTCGaatccttgcagtgtgcttgg |
| anr-DwnF-Gm | AGGAACTTCAAGATCCCCAATTCGggaagtgcacatcctcgact |
| anr-DwnR-GWR | TACAAGAAAGCTGGGTacgaagctgtccacggtcat |
| Nested PCR primers | |
| Rnd1-TnM | TATAATGTGTGGAATTGTGAGCGG |
| Rnd1-Pa1 | GGCCACGCGTCGACTAGTACNNNNNNNNNNGATAT |
| Rnd2-TnM | ACAGGAAACAGGACTCTAGAGG |
| Rnd2-Pa | GGCCACGCGTCGACTAGTAC |
| TnMSeq | CACCCAGCTTTCTTGTACAC |
| TnMRev | TGCACCGTGCAGTCGATGATAA |
| Other primers | |
| IR187-UP | GAGGGGAcCCGGgATGATCTACGACAA |
| IR187-DN | AGGTCTTCTCGGGTaccGGCGTGTTGT |
| Gm-UP | TGGAGCAGCAACGATGTTAC |
| Gm-DN | TGTTAGGTGGCGGTACTTGG |
| FabU | GGGATCCGGAATGATCTACGACAAGG |
| FabD | GGGTACCAAGTTTAGCCCGTTCATGC |
| PA1611-UP | AAATCCCTGAACTGCAGGGTTGTG |
| PA1611-DN-in | ACCTGCGCCAAGAATTCACCCATA |
| GmFRT-UP | CGAATTAGCTTCAAAAGCGCTCTGA |
| GmFRT-DN | CGAATTGGGGATCTTGAAGTTCCT |
| Anr-UP-KpnI | ATCGCGGCTGCGGTACCCTT |
| Anr-DN-BamHI | ATACAACGGATCCGCGCTGAG |
| EcglmS-DN | TGCAGCTGCTGGCTTACCATG |
| Tn7R | CACAGCATAACTGGACTGATTTC |
| DIG-fabA | DigN-ATGCGATCGATCATCAGCATGTTG |
| Bio-fadR-for | Biotin-GGACACTGTGACTTTCACCGCAACGCAACAGTCTATGACT |
| fadR-Rev | AGTCATAGACTGTTGCGTTGCGGTGAAAGTCACAGTGTCC |
| fadR-UP | GAACCGGACACTGTGACTTTCACCGCAACGCA-ACAGTCTATGACTA |
| fadR-DN | TAGTCATAGACTGTTGCGTTGCGG-TGAAAGTCACAGTGTCCG |
| 30-concatamer-for | (TAGACTGTTGCGTTGCGGTGAAAGTCACAG)3 |
| 30-biotin-rev | Biotin-CTGTGACTTTCACCGCAACGC |
a
Sequences in capital letters are common for all genes amplified in a particular experiment and overlap with the sequences of the other genes that they were spliced to, e.g. Gm or attB primer sequences. Lower-case letters indicate gene-specific sequences.