Figure 6. Beta 2-adaptin depletion induces matrix degradation in U2OS cells.

A. U2OS cells depleted of β2-adaptin and spread on collagen form structures that appear similar to invadopodia, with paxillin and actin localized to ring and core-like structures respectively. Scale bar = 20 µm. B. When plated on fluorescent 488-gelatin, β2-adaptin depleted U2OS demonstrate increased ability to degrade matrix as compared to control cells. Scale bar = 20 µm. C. Quantitation of the number of cells degrading matrix shows a significant increase in β2-adaptin depleted cells. *** = P<0.0005.